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Hello. Can you hear it? Yeah, I can. Are you able to share your slides? Yeah, we'll do that now. Brilliant. Ok. Um, so what can you see now? I can see your screen. You like you're in, you're on metal at the moment. Yeah, and that's, it's all right. It doesn't look dodgy or anything. It'll look fine once you change to uh a powerpoint side. Yeah, I can see that. Awesome. Um, but that's as a PDF. Yes. Um Do you think it's better if I Yeah, it's probably better if you use slides instead? High point? Yeah. How does that look? Yeah, that looks fine. And that's full scrapie when you do slideshow and then present, then it'll be full screen. Um, I thought I did slighter. How does that look? Yeah, that's perfect. That's working. No, cool. Um And do you need my camera? Um, that's up to you if you wanna turn your camera on, that's probably best. Sure. Ok. Thank you. And can I just check, can you see like my laser pointer? Yeah, I can see the point. Thank you. Thanks. Um You're welcome to stop. Yeah, people are joined. Yeah, we've got we've got 27 people. So OK, morning everyone. Uh my name is Sammy and today on April Fool's Day, I'm gonna be talking to you Summit one summe two integration of metabolism, cholesterol and matrix, which is quite a lot. So I've tried to keep it to like the bare bones, the essentials that you guys need to know. Um I've used a lot of kind of faculty pictures because that's what obviously comes up in the exams and um obviously it's quite a lot. So I'm gonna go through quite quickly but let me know if you want want me to slow down or anything. Um And yeah, I put my email up as well, I think. Um so that if you have any questions, just email me and I'll answer them. So yeah, let's get going with summa one. So we have your six reaction types that like cover some metabolism and I've kind of just circled the ones that come up the most often. Um And that will be covering kind of today mostly. So oxidation reduction um which is just obviously like just electron transfer eye summarization just rearranging your atoms to form different molecules, but with the same atoms and then group transfer, which is just taking the functional group of one a one molecule, shoving it onto another molecule and you get two different molecules. Ok. So glycolysis basically, as we know from like I level biology, it's an anaerobic process and it occurs in the cytosol or the cytoplasm. And that's an important distinction to note. Um It involves the investment of ATP but you get a net production of ATP at the end and your net results in glycolysis. Um really important to memorize this is you get two of everything, essentially two AP, two N A DH and two pyruvate molecules formed. Um And I've put this kind of diagram here that I found on the Note Bank um that just goes through all of the steps I thought was quite kind of aesthetic and was quite helpful for me when I was arising. I'm not gonna go through all the steps today, kind of one by one. But maybe in your own time, if you wanna have a look on the, once the slides come out, um it'd be good to have a look. OK. So I'm gonna kind of key steps of glycolysis that will probably be like examined. Um Faculty. Um Firstly, the irreversible steps. Um what, what first of which is the first step of Glyco where glucose is compared to glucose, six phosphate bihar um because it's phosphorylated, it now has a negative charge. Um And it's a different molecule. So the transporters don't recognize it anymore, it can't other cell. And so uh Gluco is now committed to like a cell metabolism essentially. Then the third step where fructose six phosphate is converted to fructose, 16 bisphosphonate uh by phosphofructokinase. And because there's a high energy difference between the reactants and the products. The ford reaction is so heavily favored that it, it's essentially just irreversible. And then the final step as well, where phosphoenylpyruvate converted is converted to pyruvate by pyruvate kinase for similar reasons to set three. But those are your irreversible steps. And when we come to the integration of metabolism, those are the steps that we need to kind of overcome to form glucon and Genesis. Um I just turned my pointer on cool other key kind of bits to know is in step three. it's kind of the most important step because it's the rate limiting step of glycolysis. Um And phosphofructokinase is known as the rate limiting enzyme. So it's an allosteric enzyme and so it almost gets inhibited by kind of negative feedback. Um once you've got a large amount of ATP um it decreases the affinity of phosphate fructi kinase or fructose six phosphate. So basically, um once you have a lot of ATP the phosphokinase will no longer bind to um fructose six phosphate and glycolysis kind of comes to a halt. And then another key kind of point to make is